Lips in 12-well plates and left for 16 h at 37 in 5 CO2. Next, they were transfected with 0.5 g of appropriate cDNA and 1.5 l of Lipofectamine2000 (Invitrogen) according to manufacturer's protocol. Two wells were transfected with the same cDNA of interest, and subsequently one was treated with NGF and the other not. Two wells on each plate were transfected with AnnexinA4, which served as a negat